Figure 1.

Nucleotide sequence alignment of target regions of published bluetongue virus (BTV) real-time reverse transcription–PCR primers and probes (in boldface), which were used for detection of Toggenburg orbivirus (TOV). A) Segment 10 (10); B) segment 1, eastern serotype specific (13); C) segment 1, western serotype specific (13); D) segment 1 (14); E), segment 5 (14). Shaded areas indicate primer and probe sequences (in sense orientation), colons indicate sequence identity, arrows indicate orientations of probes and primers, and asterisks indicate mismatches between primers/probes and TOV genome sequence.