Table 1. Real-time PCR measurement of viral sequences in birds with PPD*.

Case no.	Species†	Age/sex	PDD/ control	Primer/probe set	Organ	Ct	Virus copies‡ (300 ng total RNA)
1034	Canindae macaw	30 y/M	PDD	1034–1322	Brain	17.94	8.88 × 107
					Adrenal gland	20.51	8.77 × 106
					Proventriculus and gizzard	25.26	1.22 × 105
1322	Vinaceous Amazon	1 y/M	PDD	1034–1322	Brain	17.64	1.17 × 108
					Proventriculus	28.47	6.76 × 103
1367	Canindae macaw	30 y/F	PDD	1367	Brain	27.33	8.89 × 103
					Adrenal gland	18.92	3.87 × 106
					Proventriculus	23.82	1.13 × 105
5473	Leadbetter’s cockatoo	17 y/M	Control	1034–1322 and 1367	Brain	>36§	Negative
4858	Long-tailed parakeet	Adult/M	Control	1034–1322 and 1367	Brain	>36§	Negative
2020	Cockatiel	Young/M	Control	1034–1322 and 1367	Brain	>36§	Negative
3616	Eclectus parrot	0.25 y/F	Control	1034–1322 and 1367	Brain	>36§	Negative

*PPD, poventricular dilatation disease; Ct, cycle threshold.
†All birds were captive animals from California, USA.
‡Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
§A Ct of >36 was rated as negative based on the highest dilution of standard representing 5 copies.