Table 1. Human hMLH1-G67E mutant does not interfere with yeast MMR.
| Ratea | lys2∷InsE-A14 ±s.e.m. | Fold difference | Rate | CAN1 ±s.e.m. | Fold difference | |
|---|---|---|---|---|---|---|
| Vector only | 1.2 × 10−7 | 1.1 × 10−8 | 1.0 | 1.5 × 10−6 | 1.3 × 10−7 | 1.0 |
| hMLH1 | 2.3 × 10−3 | 1.4 × 10−3 | 18,700 | 7.7 × 10−6 | 1.2 × 10−6 | 5.1 |
| G67R | 1.5 × 10−7 | 4.0 × 10−8 | 1.2 | 8.6 × 10−7 | 3.4 × 10−7 | 0.6 |
| G67E | 2.4 × 10−7 | 1.3 × 10−7 | 1.9 | 1.7 × 10−6 | 4.9 × 10−7 | 1.1 |
MMR=mismatch repair.
Mutation rates are assessed in yeast strains expressing the various forms of human MLH1 proteins. Only wild-type hMLH1 was capable of interfering with the endogenous yeast MMR to increase mutation rates.
a
Mutation rate estimated as mutation/cell/generation.