Table 2. Mutation rates for the yeast yMLH1-G64E mutant are elevated.
| Rate a | lys2∷InsE-A14 ±s.e.m. | Fold difference | Rate | CAN1 ±s.e.m. | Fold difference | |
|---|---|---|---|---|---|---|
| WT | 1.0 × 10−7 | 1.3 × 10−8 | 1.0 | 4.2 × 10−7 | 8.0 × 10−8 | 1.0 |
| mlh1Δ | 1.9 × 10−3 | 5.8 × 10−4 | 19,000 | 1.3 × 10−5 | 1.2 × 10−6 | 30 |
| mlh1-G64R | 8.7 × 10−4 | 1.9 × 10−4 | 8,300 | 1.7 × 10−6 | 7.1 × 10−7 | 3.9 |
| mlh1-G64E | 4.4 × 10−4 | 2.7 × 10−4 | 4,200 | 3.7 × 10−6 | 2.9 × 10−6 | 8.8 |
WT=wild type.
WT yeast cells are compared with yeast cells deleted for mlh1 or expressing mutant versions of yeast mlh1. Deletion of mlh1 greatly elevated mutation rates as expected. The single-point mutation of ymlh1 of G64R or G64E also increased the mutation rates significantly.
a
Mutation rate estimated as mutation/cell/generation.