Table 1. Mutation status of cell lines used in this study.
| LKB1 | BRAF | KRAS | PIK3CA | P53 | CDNKA | EGFR | ||
|---|---|---|---|---|---|---|---|---|
| Cell line | Histology | Mutation | Mutation | Mutation | Mutation | Mutation | Mutation | Mutation |
| SKMEL28 | Malignant melanoma | c.1799T>A p.V600E | c.434_435TG>GT p.L145R | c.2257C>T p.P753S | ||||
| CAL-12T | NSCLC nos. | No proteina | c.1397G>T p.G466V | c.404G>T p.C135F | c.172C>T p.R58* | |||
| A549 | NSCLC nos. | c.109C>T p.Q37* | c.34G>A p.G12S | c.1_471del471 p.M1_*157del | ||||
| NCI-H1734 | NSCLC adenocarcinoma | c.152_153insCT p.M51fs*14 | c.37G>T p.G13C | |||||
| NCI-H460 | NSCLC large cell carcinoma | c.109C>T p.Q37* | c.183A>T p.Q61H | c.1633G>A p.E545K | c.1_457del457 p.? | |||
| NCI-H2030 | NSCLC Adenocarcinoma | c.949G>T p.E317* | c.34G>T p.G12C | c.785G>T p.G262V | ||||
| NCI-H1563 | NSCLC adenocarcinoma | c.816C>A p.Y272* | c.1_471del471 p.M1_*157del | |||||
| NCI-H2009 | NSCLC adenocarcinoma | c.35G>C p.G12A | c.818G>T p.R273L | |||||
| NCI-H1975 | NSCLC adenocarcinoma | c.353G>A p.G118D | c.818G>A p.R273H | c.205G>T p.E69* | c.2573T>G p.L858R | |||
| NCI-H1838 | NSCLC adenocarcinoma | c.818G>T p.R273L | c.1_471del471 p.M1_*157del |
Mutation status of oncogenes and tumour suppressors known to be commonly mutated in NSCLC. Mutation data taken from COSMIC (http://www.sanger.ac.uk/genetics/CGP/CellLines/).
a
No mutation has been found in this sample by sequencing; however, immunoblot analysis revealed no protein present (data not shown); NSCLC nos.=NSCLC not otherwise specified.