Figure 5.

Transcription rates have mild effects on period length at reduced temperatures. (A) Bmal1-luc fibroblasts were synchronized with dexamethasone, placed into home-made precision incubators at temperatures ranging from 31 to 41°C, and bioluminescence cycles were recorded during 168 h. Of note, the period length progressively increases with elevated temperature. (B, C) Bioluminescence cycles from untreated and α-amanitin-treated (3 μg/ml) Bmal1-luc fibroblasts were recorded for 92 h at 33 or at 37°C. At 33°C, the α-amanitin-induced period shortening was less significant (23.21±0.42 versus 23.96±0.54 h) than at 37°C (23.52±0.82 versus 26.03±0.54 h). The following P-values were obtained for data sets compared with each other: period length from cells on 3 μg/ml of α-amanitin treatment compared with untreated samples at 37°C (B) P=0.001 and at 33°C (C) P=0.12.