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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Neuron. 2008 Oct 23;60(2):308–320. doi: 10.1016/j.neuron.2008.08.012

Figure 6. RA induces local translation of GluR1 proteins.

Figure 6

(A) GluR1 mRNA was detected in neuronal dendrites as well as in glia cells. Antisense or sense probes against GluR1 mRNA (red) was used for fluorescent in situ hybridization. Neuronal soma and dendrites were labeled with MAP2 antibody (blue). Scale bar, 10 µm. (B) GluR1 and PSD-95 were enriched in, and histone H3 was selectively absent from, the hippocampal synaptoneurosome fraction relative to the whole-cell lysate. (C) Ten minute RA treatment (0.1, 1 or 10 µM) induced GluR1 synthesis in synaptoneurosomes (n = 4; *, p < 0.005). (D) GluR2, PSD-95 and RARα expression were not changed by RA treatment (n = 4, p > 0.5). (E) RA-induced GluR1 synthesis in synaptoneurosomes was blocked by cycloheximide and anisomycin, but not actinomycin D (n = 4; *, p < 0.0005). (F) Immunofluorescence staining of hippocampal sections for MAP2 (green) and RARα (red; scale bar for upper panels, 200 µm; for lower panels, 20 µm).