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. 2009 Feb 10;3(2):e377. doi: 10.1371/journal.pntd.0000377

Figure 2. Demonstration of uptake of Cy3-labeled dsRNAs or siRNA by B. malayi.

Figure 2

Adult female B. malayi (2 groups of 4 worms) were soaked in normal culture medium containing Cy3-dsRNA (0.01 mg/ml) for 24–72 h. Uptake was examined for Bm-cpl-5 dsRNA (∼800 bp) (A), Bm-cpl-5 siRNA (B), Bm-cpl Pro dsRNA (∼400 bp) (C) and Bm-cpl Pro siRNA (D). Fluorescence was visualized using a Zeiss Axiovert fluorescence microscope using the rhodamine filter set, using emission 590 nm.