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. 2009 Feb 10;3(2):e377. doi: 10.1371/journal.pntd.0000377

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Ford et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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At the end of the experiment (2 d after treatment), groups of four adult female worms were removed from culture and analyzed for differences in Bm-cpl gene-specific transcript levels by qRT-PCR. The relative amounts of Bm-cpl amplicon were determined by using the comparative C_T method and normalizing against the endogenous control gene (Bm-tub-1). The median value of the control group was set to 100% and the reduction in expression in the treated groups was calculated as a percentage of the control. ## denotes a significant difference between dsRNA or siRNA-treated worms and medium control, while *** denotes a significant difference between dsRNA or siRNA-treated worms and negative control (dsRNA of Ov-cpz-Int2) (Mann-Whitney U-test).