Table 1. GenBank accession numbers, and primer sequences and positions used for gene specific dsRNA production and real-time RT-PCR (qRT-PCR) with the resulting fragment size.
Gene target | Accession number | Primer name | Position | Primers (5′-3′) | Gene specific primer product |
dsRNA production | |||||
Bm-cpl-1 | AF331035 | Bm-cpl-1F | 548 | CCAAGTTCAGTTGATTGG | 642 bp |
Bm-cpl-1R | 1189 | GATGGGAAATGAACCCATCG | |||
Bm-cpl-5 | AY533167 | Bm-cpl-5F | 550 | CTGCCAGATCAAGTTGAC | 648 bp |
Bm-cpl-5R | 1197 | CGGGAAATGAAGCCATAG | |||
Bm-cpl Pro | AY533167 | Bm-cpl Pro F | 117 | CAATATGACGAGACTTGCGT | 334 bp |
Bm-cpl Pro R | 450 | GACCATAAATTCTTCATCGG | |||
Bm-cpz | AY533170 | Bm-cpz F | 200 | CGAAGACTTACCTATAGC | 289 bp |
Bm-cpz R | 488 | TTTCGTGTGGTATGCCAAC | |||
Ov-cpz-int2 | AY591516 | Int2 F | 621 | TTCCTTTTCGGAGAATTAGC | 512 bp |
Int2 R | 1132 | TATGGAAAAAGATCGAAATT | |||
qRT-PCR | |||||
Bm-tub-1 | AY705382 | Bm-tubF | 173 | AATATGTGCCACGAGCAGTC | 307 bp |
Bm-tubR | 479 | GGATACTCCTCACGAATTT | |||
Bm-cpl | AY533167 | Bm-cpl RTF | 100 | GACAAAGATTACAAACAGGGC | 381 bp |
Bm-cpl RTR | 480 | TGATTGGGCAGTCGAAGTC |