Skip to main content
. 2009 Feb 3;7(2):e1000029. doi: 10.1371/journal.pbio.1000029

Figure 2. EM-GRN Factors Induced by the Pmar1-Dependent Endomesoderm Induction.

Figure 2

(A) In all cases shown in (B–D), two-color FISH was used to detect ectopic induction of test transcripts (green) adjacent to gfp (red)-mRNA–expressing blastomeres in embryos injected with 0.02 μg/μl SpHE-gfp and 0.015 μg/μl SpHE-pmar1 (e–h in each case) compared to embryos injected with 0.02 μg/μl SpHE-gfp alone (a–d in each case).

Test transcripts: (B) z13 expression at 16–18 h p.f. (C) foxA expression at 20 h p.f.

(D) eve expression at 14 h p.f. Embryos shown are representative of over 80% of at least 50 embryos in each of three separate experiments in which gfp mRNA was detected in nonvegetal regions.

Black scale bar in (B) d represents approximately 20 μm. DIC images in (B) d and h, (C) d and h, and (D) d and h illustrate the absence of detectable developmental defects in injected embryos. gfp-expressing cells containing Pmar1 in (C) g and h have adopted a mesenchyme phenotype and ingressed into the blastocoel at 20 h p.f.