Skip to main content
. 2009 Feb 3;7(2):e1000029. doi: 10.1371/journal.pbio.1000029

Figure 3. EM-GRN Factors That Are Not Induced by Ectopic Pmar1 Expression.

Figure 3

(A) In all cases shown in (B–D), two-color FISH showed the absence of ectopic induction of test transcripts (green) adjacent to gfp-mRNA–expressing (red) blastomeres in embryos injected with 0.02 μg/μl SpHE-gfp and 0.015 μg/μl SpHE-pmar1 (e–h in each case) compared to embryos injected with 0.02 μg/μl SpHE-gfp (a–d in each case).

Test transcripts:

(B) blimp1 expression at 14–16 h p.f.

(C) wnt8 expression at 14–16 h p.f.

(D) brachyury expression at 20 h p.f.

Embryos shown are representative of 100% of at least 50 embryos in each of three separate experiments in which gfp mRNA was detected in nonvegetal regions. DIC images in (B ) d and h, (C) d and h, and (D) d illustrate the absence of detectable developmental defects in injected embryos. gfp-expressing cells containing Pmar1 in (D) panels g and h have adopted a mesenchyme phenotype and ingressed into the blastocoel at 20 h p.f.