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. 2009 Feb 3;7(2):e1000029. doi: 10.1371/journal.pbio.1000029

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Embryos were treated with either (a and b) DMSO (Control) or (e and f) SB-431542 (5 μM) and analyzed by WMISH to detect (a and e) gcm at 18 h and (b and f) gataE at 18 h. The same mRNAs were detected in embryos injected either with (c and d) buffer or (g and h) ActivinB MO1 (1.2 mM) at 18 h (c, g, d, and h). The accumulation of each of these transcripts in veg2 secondary mesoderm precursors was independent of ALK4/5/7 (e and f vs. a and b) and ActivinB (g and h vs. c and d) function at the stages assayed. All images are representative of the molecular phenotypes observed in more than 80% of a minimum of 50 embryos in each of three separate experiments.

(B) At 34 h p.f., (a–c) buffer-injected controls contain 29 gcm-mRNA expressing (n = 5 embryos examined) cells (green in b and c) compared to 40 (n = 5 embryos examined) such cells (green in e and f) in embryos injected with (d–f) 1.2 mM ActivinB MO1. Nuclei are stained with DAPI (blue).