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. 2009 Feb 3;7(2):e1000029. doi: 10.1371/journal.pbio.1000029

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) SoxB1 protein (green), gcm mRNA (red), and DAPI nuclear staining (blue) at 18 h p.f.: (a–c) buffer-injected controls; (d–f) 1.2 mM ActivinB MO1–injected embryos; and (g–i) 0.15 mM Delta MO-injected embryos. Images represent the phenotype observed in over 80% of at least 50 embryos in each of three separate experiments. SoxB1 protein clears normally from veg2 secondary mesoderm precursors in ActivinB (d–f vs. a–c) and Delta (g–i vs. a–c) morphant embryos.

(B) (a–c) SoxB1 protein (green), z13 mRNA (red), and DAPI nuclear staining (blue) at 8 h p.f. in normal embryos showing high levels of SoxB1 protein in nuclei of z13-expressing cells at the cleavage stage of development. The optical section is slightly oblique with respect to the animal-vegetal axis, and z13-expressing cells are at the bottom.