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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Dev Biol. 2008 Dec 10;326(2):347–356. doi: 10.1016/j.ydbio.2008.11.035

Figure 2. hidw138 is new allele of hid.

Figure 2

(A) Schematic representation of the hid locus. An approximate 60Kb region surrounding the hid locus is shown. A solid black arrow represents the hid transcript unit. The black inverted triangle represents the location of the P-element insertion in the hidPZ line. The hid ORF is shown by shaded boxes and the 5’ and 3’ UTR are shown by open boxes. (B) PCR product of hid exon2-4 from different genomic DNA. Two different size PCR fragments were obtained from w138/w300 transheterozygotes, a single fragment corresponding to the shorter one was obtained from w138 homozygote. (C) The sequence of w138 mutation. Amino acids sequence is shown beneath the nucleotide sequence. Deleted 312bp DNA sequence in the second exon is highlighted in gray. This deletion introduced a frame shift leading to the coding of 13-amino-acids followed by a stop codon. (D) Schematic representation of open reading frames of wild-type Hid and Hidw138.