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. 2009 Feb 6;4(2):e4365. doi: 10.1371/journal.pone.0004365

Figure 6. Blockade of 3-MA-dependent vesicular transport by L. casei might induce the degradation of IP-10.

Figure 6

(A) Mode-K cells were stimulated with TNF (10 ng/ml) alone or together with L. casei (moi 20) in the presence or absence of 3-MA for 24 h and the amount of IP-10 in the cell culture supernatant as well as intracellularly was analyzed by ELISA and Western blot. Figure A is representative for three independent experiments and bars represent mean values (+/− SD) of triplicate samples. (B) The scheme illustrates our hypothesis that L. casei might target 3-MA dependent vesicular transport of IP-10 resulting in a secretional blockade and subsequent degradation of the chemokine.