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Kinesin ATPase assay using CENP-E purified motor domain protein. ATPase activity was measured by generation of Pi following addition of ATP and taxol-stabilized MTs to purified kinesin motor protein. Bkg- Background; no ATP added, No MTs- no microtubules added to reaction, DMSO-negative control, no UA62784 added. Error bars are shown to indicate standard deviation (n=4).
