Skip to main content
. Author manuscript; available in PMC: 2009 Feb 2.
Published in final edited form as: Mol Cancer Ther. 2008 Apr;7(4):961–971. doi: 10.1158/1535-7163.MCT-07-0470

Figure 5.

Figure 5

MKK6 activates NAG-1 promoter in a p38 kinase-dependent mechanism. A, PC-3 cells were cotransfected with NAG-1 promoter reporter plasmid (−966/70) and pcDNA3.1-lacZ or pcDNA3-MKK6b(E). As an internal control, the phRL-null vector was used to correct for transfection efficiency. Twenty-four hours after transfection, medium was changed and cells were incubated for another 24 h in the presence or absence of 20 μmol/L SB203580. Luciferase activity was measured 48 h after transfection and firefly luciferase activity was normalized to Renilla luciferase. B, PC-3 cells were cotransfected with indicated length of NAG-1 promoter reporter plasmid and pcDNA3.1-lacZ or pcDNA3-MKK6b(E). As an internal control, the phRL-null vector was used to correct for transfection efficiency. Luciferase activity was measured 48 h after transfection and firefly luciferase activity was normalized to Renilla luciferase.