Table 3.

Aminoglycoside-induced inhibition of luciferase synthesis (IC₅₀, μM)

Aminoglycoside	A-site rRNA
	Bacteriala	Mitochondrial (mt) hybrid	mt A1555G hybrid	mt C1494T hybrid
Neamine	1.4	131	32	35
Neomycin B	0.04	0.7	0.5	0.4
Paromomycin	0.03	33	2.4	3.1
Kanamycin A	0.05	15.7	1.2	1.1
Tobramycin	0.02	7.8	0.8	0.9
Amikacin	0.02	7.0	0.4	0.6
Gentamicin	0.03	5.7	0.6	0.7
Netilmicin	0.05	17.6	0.8	2.6

IC₅₀ values represent the drug concentrations in μM that are required to inhibit synthesis of functional firefly luciferase to 50%. Relative luciferase activity plotted against aminoglycoside concentration is shown in Fig. 2C.

^aM. smegmatis wild-type rRNA