Fig. 4.

Recruitment of recombinant AMPAR subunits at Nrx1β-Fc beads and synapses. Neurons were transfected with Nlg1 and PSD-95-mCherry plus either GluR1-GFP or GluR2-GFP, incubated for 4 h with Nrx1β-Fc beads with or without TTX/APV, then processed for live anti-GFP staining. Examples of recombinant GluR1 (A) or GluR2 (B) stainings at Nrx1β-Fc beads (arrows) and nearby endogenous synapses, as identified by PSD-95-mCherry clusters (arrowheads). Quantification of the enrichment factor, i.e., the fluorescence signal on a bead (C) or synapse (D) divided by the neurite level, for all conditions. The number of beads or clusters quantified is given in each column. Data were analyzed by one-way ANOVA and compared by Tukey test (***, P < 0.0002).