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. 2008 Dec 15;105(52):20918–20923. doi: 10.1073/pnas.0809147105

Fig. 2.

Fig. 2.

Hyphae-inducing conditions permit growth when expression of PES1 is repressed from tetO. Strains were grown overnight in YPD without doxycycline at 30 °C and diluted to an initial density of OD600 0.2. Fivefold dilutions were spotted onto YPD (A), YPD with 20 μg/ml doxycycline (B), Spider (C), Spider with 20 μg/ml doxycycline (D), 20% calf serum 2% glucose (E), and 20% calf serum 2% glucose with 20 μg/ml doxycycline (F). Plates were incubated at 37 °C for 4 days. (1) JKC915 (HIS1/his1::FRT-tetR PES1/PES1). (2) JKC956 (HIS1/his1::FRT-tetR PES1/pes1::FRT). (3) JKC962 (HIS1/his1::FRT-tetR PES1/pes1::FRT). (4) JKC1137 (HIS1/his1::FRT-tetR pes1::FRT/FRT-tetO-PES1). (5) JKC1143 (HIS1/his1::FRT-tetR pes1::FRT/FRT-tetO-PES1).