Catalytic efficiencies of different BcII forms. Relative catalytic efficiencies of G262S BcII, N70S BcII, and N70S/G262S BcII compared with WT BcII against benzylpenicillin (PEN), nitrocefin (NTR), imipenem (IMI), cefotaxime (CTX), and cephalexin (CLX). The relative activity is the ratio of the mutant's kcat/KM value to that of WT. y axis is in log scale.