Fig. 3.

T regs from VDR KO mice are functionally normal. CD4⁺/CD25⁻ and CD4⁺/CD25⁺ T cells were isolated from VDR KO and WT mice. (A) WT CD25⁻ proliferation was inhibited equally by WT and VDR KO CD4/CD25⁺ cells at all ratios tested. (B) VDR KO CD25⁻ proliferation was inhibited equally by WT and VDR KO CD4/CD25⁺ cells at all ratios tested. (C) VDR KO T regs suppress IBD in the Rag KO mouse. The percentage change in BW of Rag KO mice that did not receive any cells (control, CTRL), received only WT CD4/CD25⁻ T cells (CD25⁻), received WT CD4/CD25⁻ and WT CD4/CD25⁺ (WT T reg), or received WT CD4/CD25⁻ and VDR KO CD4/CD25⁺ (VDR KO T reg) is shown. Only Rag KO recipients of CD25⁻ cells lost significantly more BW by the end of the experiment than the CTRL, WT T reg, or VDR KO T reg Rag KO recipients, P < 0.05.