Table 1.

Production profile of alcohols from the designed pathway, with different KIVD mutants

Note that the V461A/F381L mutant gives the highest titer of 3-methyl-1-pentanol. E. coli cultures were grown in M9 medium with 20 g/L glucose plus 0.1 mM IPTG at 30 °C for 40 h. These products were identified by GC-MS and quantified by GC-FID. ND, not detectable.