Table 1.
Effects of erlotinib on cell cycle in KI cells
| % Cells in phase |
P | % Cells in phase |
P | |||||
|---|---|---|---|---|---|---|---|---|
| Vehicle |
Erlotinib 0.1 μM |
Erlotinib 0.5 μM |
||||||
| Mean | SD | Mean | SD | Mean | SD | |||
| hTERT-HME1 WT | ||||||||
| G1 | 71.6 | 6.6 | 77.4 | 1.0 | 0.208 | 77.0 | 1.2 | 0.227 |
| G2/M | 14.2 | 2.2 | 11.1 | 1.3 | 0.047 | 11.4 | 1.3 | 0.022 |
| S | 14.7 | 3.9 | 11.5 | 2.1 | 0.309 | 11.6 | 2.3 | 0.349 |
| G2 + S | 28.9 | 5.9 | 22.6 | 1.0 | 0.152 | 23.0 | 1.2 | 0.167 |
| Sub-G1 | 1.0 | 0.3 | 0.3 | 0.1 | 0.013 | 0.3 | 0.2 | 0.061 |
| KI EGFR delE746-A750 | ||||||||
| G1 | 70.3 | 3.0 | 87.3 | 2.2 | 0.006 | 85.3 | 1.5 | 0.007 |
| G2/M | 16.4 | 2.6 | 9.2 | 1.6 | 0.038 | 10.7 | 1.2 | 0.046 |
| S | 13.3 | 2.6 | 3.4 | 0.6 | 0.004 | 4.0 | 0.8 | 0.010 |
| G2 + S | 29.7 | 3.0 | 12.7 | 2.2 | 0.006 | 14.7 | 1.5 | 0.007 |
| Sub-G1 | 1.6 | 1.9 | 3.2 | 2.5 | 0.307 | 3.7 | 3.7 | 0.517 |
WT and EGFR KI cells were incubated for 48 h with the indicated concentrations of erlotinib, and the effect on cell cycle was assessed by FACS analysis. Erlotinib induced a significant arrest of EGFR KI clones in the G0/G1-phase of the cell cycle, while minimally affecting parental cells. Means of at least four independent experiments are shown. Significance by paired t test was taken at P < 0.01.