Fig. 3.

DC0/+ mutants do not increase LTM although they do increase ARM in a different genetic background. (A) LTM, a second consolidated form of memory that forms after 10 spaced trainings, is not increased in DC0/+ mutants. LTM measured 4 days after spaced training remains unchanged relative to wild-type in DC0^B3/+ and DC0^H2/+ flies (P = 0.9629 and P = 0.5730 as assayed by t test). n = 10 and 11, respectively. (B) Memory retention curves of wild-type and DC0^B3/+ flies after spaced training. Memory is improved 1 day after training but returns to normal 4 days after training in DC0^B3/+ flies. Two-way ANOVA demonstrates significant differences caused by retention time (P < 0.0001) and interaction between genotype and retention time (P = 0.0142). Bonferroni post hoc analyses indicate that DC0^B3/+ shows significant differences from wild type at a retention time of 1 day (P < 0.01) but not at 4 and 6 days (P > 0.05). n = 5–10 for each data point. (C and D) Improvement of memory in DC0^B3/+ mutants is not caused by specific background effects. Both 3-h memory after single-cycle training (C) and 24-h memory after massed training (D) are improved by the DC0^B3/+ mutation in a w1118 background. n ≥ 6. **, P < 0.01; ***, P < 0.001. N.S., not significant.