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Kinetic parameters for ATP of autoinhibited titin kinase (WT and K36A) and the constitutively activated WT-kin3 measured by AFM or in solution
| Construct | kon, 1/M·s (AFM) | koff, 1/s (AFM) | Kd, μM (AFM) | KM, μM (solution) | Autophosphorylation |
|---|---|---|---|---|---|
| WT | 1.8 ± 0.3 × 104 | 6 ± 3 | 347 | No activity | − |
| K36A | 2.3 ± 0.5 × 104 | 41 ± 11 | 1,810 | No activity | − |
| WT-kin3 | 270 | + |
