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. 2009 Feb 9;4(2):e4398. doi: 10.1371/journal.pone.0004398

Figure 8. SWCNT functionalized with PS but not with PC engulfed in vivo by murine alveolar macrophages.

Figure 8

A. a)Transmission electron micrographs of bronchoalveolar macrophages isolated from C57BL/6 mice exposed to PS-coated SWCNT; b) high magnification image of the area shown in (a). B. Alveolar macrophages preferentially phagocytoze PS-coated SWCNT in vivo. TEM images were used to calculate the percent of phagocytosis-positive macrophages. At least 150 cells were counted for each treatment. Data are mean±s.d., *p<0.05, PS-coated SWCNT vs non-coated or PC-coated SWCNT. C57BL/6 female mice, 7–8 weeks old, were exposed to SCWNT (40 µg/mouse) by pharyngeal aspiration. Twenty-four hours following exposure, mice were sacrificed using sodium pentobarbital. Mice were lavaged with sterile PBS. The lavage fractions were pooled and centrifuged to obtain the cellular fraction. Cells were then fixed for TEM.