Table 1.

Summary of cell/tissue characteristics following introduction of siGLO/siRNA sequences via a number of different methods.

Cell type	Analysis	No Reagent	Lipid-Based transfection	Nucleofection
				Cell Line Solution L	Cell Line Solution V
BeWo	Transfection efficiency (% cells containing siGLO)	0%	Lipofectamine 2000: 50% Oligofectamine: 40% Fugene HD: <40%	Prog A020: 60% Prog T020: 50% Prog X001: >70% Prog X005: >90%	Prog A020: 60% Prog T020: 50% Prog X001: >70% Prog X005: >70%
	Cell Survival (%)	85%	Lipofectamine 2000: 40% Oligofectamine: 70% Fugene HD: 70%	Prog A020: 50% Prog T020: 50% Prog X001: 60% Prog X005: >70%	Prog A020: 50% Prog T020: 50% Prog X001: 60% Prog X005: 60%
	PLAP mRNA (%)	N/A	N/A	Prog X005: 75%	N/A
	PLAP protein (%)	N/A	N/A	Prog X005: >70%	N/A
				Basic Mammalian Epithelial Cell Solution
Primary trophoblast	Transfection efficiency (% cells containing siGLO)	50%	Lipofectamine LTX: 80% DharmaFECT2: 95%	Prog S005: Most of surviving cells Prog T020: Most of surviving cells Prog T023: Most of surviving cells Prog U017: Most of surviving cells
	Cell Survival (%)	85%	Lipofectamine LTX: 40% DharmaFECT2: 85%	Prog S005: <30% Prog T020: <30% Prog T023: <30% Prog U017: <30%
	PLAP mRNA (%)	N/A	DharmaFECT2: 60%	N/A
	PLAP protein (%)	N/A	>70%	N/A
1st Trimester placental tissue	Transfection efficiency (% cells containing siGLO)	100% syncytium 0% cytotrophoblast 0% stromal cells	Lipofectamine 2000, Oligofectamine, Fugene HD all gave similar transfection efficiencies:100% syncytium; 0% cytotrophoblast; 0% stromal cells	Prog X005: 100% syncytium; 70% cytotrophoblast; 30% stromal cells Prog X001: 100% syncytium; 50% cytotrophoblast; <10% stromal cells Prog U017: 100% syncytium; 50% cytotrophoblast; 10% stromal cells Prog U007: 100% syncytium; 80% cytotrophoblast; 40% stromal cells
	Cell Survival (%)	>90%	N/A	Prog U007: >70%
	PLAP mRNA (%)	N/A	N/A	Prog U007: 85%
	PLAP protein (%)	<20%	N/A	Prog U007: >60%