Double immunoperoxidase staining with antibodies to p65, p50, IKKα and α-SM actin in serial paraffin sections of chicken embryo aorta at day E14 of development. Nuclear immunoreactivity for p50 or p65 (brown) is observed in some mesenchymal and lamellar cells (arrows) that also exhibit cytoplasmic immunoreactivity for α-SM actin (red). For IKKα (brown), immunoreactivity is detected colocalizing with α-SM actin (red) in the cytoplasm of some mesenchymal and lamellar cells (arrows). L, lumen. e, endothelium. mc, mesenchymal cells. lc, lamellar cells. Scale bar = 20 µm.