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Quantitative analysis of ACh effects on individual thalamic fibers and growth cones. Application of 1 mM and 10 mM ACh to the culture medium decreased thalamic fiber length. At the same time growth cone size was increased almost up to 50% at both ACh concentrations used. n = number of thalamic axons/growth cones analyzed; ***p ≤ 0.0001; *p ≤ 0.01; n.s. = not significant. Asterisk in the bars refer to the control condition.
