Figure 9.

Actin, IAA, PIN1, and RGII labelings in root apices of wild-type and maize mutants. Actin (A, F, K, P, U), IAA (B, G, L, Q, V), PIN1 (C, H, M, R, W), and RGII (D, I, N, S, X) and RGII after two hours of BFA-treatment (E, J, O, T, Y) labelings in stele periphery cells of the transition zone the wild-type (A–E), semaphore1 (F–J), lrt1 (K–O), rum1 (P–T), and lrt1/rum1 (U–Y) mutants. Note the depletion of F-actin and IAA from the cellular end-poles, which is correlated with small size of BFA-induced compartments (E, J, O, T, Y) but PIN1 shows still a signal on the end-poles. The only exeption is the lrt1 mutant which is, in contrast to all other mutants, also not affected in PAT.⁴⁴ Bars: A,C,F,K, N, P, S, U and X, 8 µM; B, D, E, G, H, I, J, L, M, O, Q, R, T, V,W and Y, 10 µM.