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. 2006 May-Jun;1(3):122–133. doi: 10.4161/psb.1.3.2759

Table 1.

Assay parameters of IAA-N-antibodies in ELISAs

Antibody Characteristic in ELISA Antibody No. 156
Titre 1:1250
Unspecific binding 3.5%
Midrange (B/Bo = 50%) 360 pmol
Detection limit 35 fmol, 8 ng
Linear range of measurement 22–5000 fmol
Intraassay variability 5.6%
Interassay variability 7.4%
Amount of tracer per assay 16.7 ng