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SGK1 is activated by active Akt1. Wild-type (WT) or kinase-dead (KD) ΔSGK1 was coexpressed with Myc-PDK1, HA-Akt1, or HA-Myr-Akt1 in HeLa cells. Protein expression was probed with anti-Myc, anti-FLAG, and anti-HA antibodies (upper two panels). Immunoprecipitated ΔSGK1 was assayed with GST-Nedd4-2 as substrate; shown are an autoradiogram and quantitation of three experiments (lower panel, plotted comparing wild-type SGK1 and kinase-dead ΔSGK1).
