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. 2009 Feb 6;284(6):3537–3545. doi: 10.1074/jbc.M807001200

FIGURE 1.

FIGURE 1.

Lack of EC-SOD leads to significant increases of syndecan-1 in bronchoalveolar lavage fluid (BALF) and lung tissue after asbestos or bleomycin exposure. Syndecan-1 in the BALF and lung homogenates of wild type and EC-SOD KO mice was detected by Western blot analysis and presented as normalized net intensity (mean ±S.E.), n = 5 mice per group. Results for the BALF data are standardized to protein loading as determined by Ponceau red staining of the membrane. Results from lung homogenates are standardized to β-actin. Shed syndecan-1 in the BALF at: (A) 1-day post-asbestos exposure, *, p < 0.05; (B) 14-days post-asbestos exposure, *, p < 0.05; (C) 28-days post-asbestos exposure, *, p < 0.05, **, p < 0.001; and (D) 7-days post-bleomycin exposure, *, p < 0.001. E, syndecan-1 in lung homogenates at 14-days post-asbestos exposure and (F) 28 days post-asbestos exposure, *, p < 0.05. Co-treatment of EC-SOD KO mice with asbestos and purified EC-SOD results in G–a decreased neutrophils, *, p < 0.001; **, p < 0.05; and in G-b decreased levels of syndecan-1, *, p < 0.05; **, p < 0.01, in the BALF at day 1, n = 4.