FIGURE 6.

Specific binding of the heparan sulfate proteoglycan form of epithelial CD44v3 to CD11b/CD18 via its decorating heparan sulfate moieties. A, binding curve of purified CD44v3 to β₂-integrins and BSA. B, binding of CD11b/CD18 to immobilized CD44v3 before and after treatment with various deglycosidases, including heparinase I, II, and III (Hep. I-III), chondroitinase ABC (Chondr.), and neuraminidase (Neurami.). C, binding of CD44v3 to CD11b/CD18 in the presence or absence of various inhibitors, including antibody C3H7, C1F3, 44a, and anti-JAM-C at 20 μg/ml each, FBG (50 μg/ml), heparin sulfate (HS) (150 μg/ml), and 3 mm EDTA. The data are presented as the means ± S.D. (n = 3).