Figure 5.

Donor GFP⁺ cells are not localized in the hair follicles of the skin. Immunofluorescence staining of the skin of the animals treated with GFP⁺CD150⁺CD48⁻ BM infusion shows that GFP⁺ donor cells have localized outside of the hair follicles. Donor cells were visualized using anti-GFP antibody (green, top panel), and hair follicles were visualized using an anti–cytokeratin 5 antibody (red, middle panel). Donor cells and hair follicles are shown (vertical and horizontal arrows, respectively) on the image merged with blue DAPI nuclear stain (bottom panel).