Table 2.
Relative expression of murine col7 RNA as assessed by quantitative RT-PCR
| Sample | Cell type | Col7 expression |
|---|---|---|
| 1 | Wild-type whole BM | 1.00 |
| 2 | Wild-type EpiSC | 4034.01 (± 2.0) |
| 3 | Wild-type TAC | 911.44 (± 1.26) |
| 4 | Wild-type skin | 502.86 (± 0.8) |
| 5 | Wild-type MAPC | 275.71 (± 0.02) |
| 6 | Wild-type MSC | 15.68 (± 0.1) |
| 7 | Col7a1−/− MSC | 0 |
| 8 | Wild-type BM CD150+48− | 0.92 (± 0.18) |
| 9 | Wild-type BM Lin+ | 0 |
| 10 | Wild-type BM Lin− | 1.37 (± 0.38) |
| 11 | Wild-type BM CD150− | 0 |
All assays were performed in duplicate and normalized to wild-type murine BM using the 2−ΔΔCT method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression served as the control gene.
BM indicates bone marrow; EpiSC, epidermal stem cell; TAC, transient amplifying cell; MAPC, multipotent adult progenitor cell; and MSC, mesenchymal stem cell.