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NCAM siRNA-mediated interference. A, endogenous NCAM protein was markedly reduced by NCAM siRNA. B, NCAM siRNA greatly reduced activation of NF-κB by TNF-α in HSG cells, in comparison with NCAM scrambled siRNA transfection. NCAM siRNA had no effect on constitutive NF-κB activity in HSG cells. The relative luciferase activities are shown as -fold induction compared with the activity of untreated samples. Each column and bar represents the mean ± S.E.M. of three independent experiments.
