Figure 1.
Effects of voluntary exercise on protein levels of E-cadherin and nuclear β-catenin in small intestinal tumors and normal small intestine of ApcMin/+ mice on AIN93G diet. Samples from Experiment 1. A. Western blot analyses of postnuclear (for E-cadherin) and nuclear (for β-catenin) fraction of 6 representative tumor samples (out of a total of 9 samples analyzed) per group. Tumors used for these analyses were ~1 mm in the largest diameter. B. Protein levels were quantified by using Adobe Photoshop software (normalized by levels of β-actin for E-cadherin and levels of histone H3 for nuclear β-catenin). Columns: mean values (in arbitrary units) of the number of mice (9 per group); bars: SE; *: p < 0.03 by two-tailed t-test. C. Western blot analyses of postnuclear (for E-cadherin) and nuclear (for β-catenin) fraction of 4 representative normal small intestine samples per group.