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. 2008 Dec 19;9:618. doi: 10.1186/1471-2164-9-618

Table 2.

Microsatellite markers' characteristics and genetic variability

Marker Primer pairs E.S Tm Samples N na ne Ho He HWEG2
Boms2 F: GCTGTTTTGAATGTCAGCATC
R: TGTATGCGTGACTGTTTACG		 128 50 wild
laboratory		 20
13		 3
3		 1.75
2.15		 0.45
0.54		 0.44
0.56		 -
-
Boms3a F: CAGTCGCCCTTTAATTTGC
R: GGGTCCTTTTGTTCTCAGG		 176 50 laboratory 32 4 2.39 0.63 0.59 -
Boms3b F: AGGACCCTGGCACAATTCG
R: TATGGCATGGCAAGCAGC		 171 50 laboratory 32 4 2.26 0.59 0.57 -
Boms5 F: TCTCGCCCCAATTACCAC
R: GAATTTTGGCAACATCCAAGC		 105 50 laboratory 34 4 3.25 0.79 0.70 -
Boms6a F: TCACTAAAAGGAGTCCGCAC
R: GAGCAGGTCAGAGGCAAAAG		 173 50 laboratory 35 3 1.64 0.43 0.39 -
Boms6b F: AAACCTTACCCTTTTGCCTC
R: AGTGCCAACTGAATGCTG		 118 50 laboratory 35 3 2.10 0.49 0.53 -
Boms8 F: TGACATACATGCCTTCATTCAC
R: CAGAAAAGCTTAAAACTAGCGG		 75 50 wild
laboratory		 20
9		 6
3		 3.10
2.66		 0.85
0.56		 0.69
0.66		 -
-
Boms10 F: CAGAGCATCTCGCTTTGG
R: TCAACAATCCCAGCAAAATC		 172 50 wild
laboratory		 20
33		 5
2		 3.33
1.27		 0.70
0.12		 0.72
0.22		 -
+
Boms11 F: ATAGGCATTGGCAGCGAAG
R: CACAGTGGGCCGAAATCAC		 185 50 wild
laboratory		 20
25		 4
2		 1.88
1.68		 0.50
0.32		 0.48
0.41		 -
-
Boms12 F: CGCGTTTTCATACTTTAACACC
R: TTCATTTGGCCTTTGTGC		 158 50 wild 19 3 1.17 0.16 0.15 -
Boms14 F: TTTGTAATTCGCAGAAGGCAC
R: AGGAGGACTGACAGAAGGACAC		 147 50 wild 8 4 2.72 0.38 0.68
Boms16 F: CAGACAATGGATGGATACATGC
R: GGAGAAGTCAAATTGTGACAGC		 109 50 wild 20 5 1.72 0.50 0.43 -
Boms17 F: ATTAGACCATAGTGTTCTCAC
R: AAGATGTTGAGTGCCGTTG		 170 50 wild
laboratory		 20
31		 7
5		 5.76
1.41		 0.35
0.19		 0.85
0.29		 +
-
Boms18 F: GCCATGAATGCAGACCAC
R: CCTATTCAAATGCACGCAAAAC		 171 50 wild
laboratory		 20
33		 6
3		 2.56
1.94		 0.80
0.39		 0.63
0.49		 -
+
Boms21 F: TCGCCTCTTACCTCACAACC
R: ACCATCCTTAGTCAGCACAGTC		 188 50 wild
laboratory		 20
28		 6
4		 3.77
1.16		 0.75
0.14		 0.75
0.14		 -
-
Boms22 F: GTAAAGCACACGGAAGCG
R: TGAGGTCAAAAAGGATGCTAAG		 211 50 wild
laboratory		 18
7		 2
2		 1.06
1.32		 0.06
0.29		 0.06
0.26		 -
-
Boms24 F: ATTTCGCTTGCCACAAAC
R: CGCCCAAGCACTTAAAAC		 215 50 laboratory 33 2 1.77 0.39 0.44 -
Boms25 F: TGGAATGCGCTATTTTGTTG
R: ACTCGTATATACGTACATGG		 167 50 wild
laboratory		 20 33 5
3		 3.49
2.16		 0.80
0.55		 0.73
0.55		 -
-
Boms27 F: CGACTTGAAGGACAATTGG
R: GGCGTGAGTAGTTTCTATAAGC		 129 50 wild
laboratory		 20
10		 5
3		 2.02
2.41		 0.55
0.50		 0.52
0.62		 -
-
Boms29 TGAAGGTGATGAATGAAAGC
GGAATGACTGTGAGCAAGC		 118 50 wild
laboratory		 20
13		 5
1		 2.57
1.00		 0.60
0.00		 0.63
0.00		 -
Boms30 F: CTGACTTCTTGCTTTACACG
R: CAGCTTATCTGCTTTAAGTGC		 150 50 wild
laboratory		 20
9		 4
4		 2.12
2.19		 0.70
0.78		 0.54
0.58		 -
Boms31 F: TGCTTGAGTTGCTCGTTGG
R: GCCGCATGACATAAAGAATCG		 173 50 wild
laboratory		 20
30		 4
2		 3.27
1.03		 0.75
0.03		 0.71
0.03		 -
Boms32 F: TGTATGTATTTGTGCGTCG
R: GCTTAGACCATTTGCTCC		 125 50 wild 20 7 3.96 0.55 0.77 -
Boms34 F: ACGCCGCACACTTCTTAAAC
R: CACCCAACTTTTGTAGTTTCC		 219 50 laboratory 34 3 2.08 0.65 0.53 -
Boms47 F: CAAACACACGCTAAAACG
R: TTTAACCCAGAGGCTTGC		 158 50 wild 18 6 3.15 0.61 0.70 -
Boms58 F: AGTTGGACGCGCACATATC
R: AGCGCGTACGAGCTTTAGC		 181 50 wild
laboratory		 18
30		 7
3		 5.02
1.15		 0.72
0.13		 0.82
0.13		 -
-
Boms59 F: AGCGCTTACATAAATATAGCTAC
R: TCCCCGTAAAGCCATAAAGTC		 171 50 wild 20 5 2.27 0.50 0.57 -
Boms60 F: TGGACGCGCACATATCAG
R: ACGACGTTTAGCGGAAATGAG		 170 50 wild
laboratory		 20
37		 6
3		 3.14
1.21		 0.70
0.19		 0.70
0.18		 -
-
Boms62 F: CTTTCGCTGCCTCCATTTG
R: CAAAACCCCTCTGCAATCC		 174 50 wild 20 2 2.00 0.55 0.51 -
Boms64a F: TGCTAGGCTGAACATTCG
R: TGTTTTGCTGTTTCCAGG		 129 50 wild 20 Multiple bands
Boms64b F: TGGAAACAGCAAAACACC
R: AGCGAATCAAGAGACAGC		 137 50 wild 10 Multiple bands
Medflymic9 Stratikopoulos et al., submitted 50 wild 8 3 2.42 0.88 0.63
Medflymic142 >> 50 wild 9 monomorphic
Medflymic149 >> 50 wild 8 monomorphic
Medflymic150 >> 50 wild 8 3 2.03 0.50 0.54
Medflymic151 >> 50 wild 20 5 2.66 0.35 0.64 +
Medflymic152 >> 50 wild 20 6 5.00 0.40 0.82 +
Medflymic153 >> 50 wild 20 3 2.24 0.40 0.57 -
Medflymic154 >> 50 wild 10 monomorphic
Medflymic157 >> 50 wild 10 monomorphic
Medflymic158 >> 50 wild 8 3 2.25 0.63 0.59
Medflymic163 >> 50 wild 10 monomorphic
Medflymic22 >> 55 wild 20 Multiple bands
Medflymic23 >> 55 wild 20 monomorphic
Medflymic29 >> 55 wild 20 Multiple bands
Medflymic40 >> 55 wild 20 Multiple bands
Medflymic61 >> 55 wild 20 4 2.32 0.45 0.58 -
Medflymic64 >> 55 wild 20 monomorphic
Medflymic72 >> 55 wild 20 monomorphic
Medflymic109 >> 55 wild 20 2 1.05 0.05 0.05 -
Mean wild (monomorphic excluded)
Mean laboratory (monomorphic excluded)		 19.5
26.5		 4.63
3.14		 2.74
1.90		 0.53
0.44		 0.57
0.44

E.S: Expected size

Tm: PCR annealing temperature

N: sample size

na: actual number of alleles; ne: effective number of alleles

Ho: heterozygosity observed; He: heterozygosity expected

(-): in HWE; (+): out of HWE