Table 4.
Conservation of Bactrocera oleae microsatellite markers in Tephritidae
| Species/ Genera |
Functional primer pairs | Expected size | Presence of SSR motif | Presence of the expected motif | Mean number of uninterrupted repeats |
| B. oleae | 29 | 29 | 29 | 29 | 100% |
| B. correcta | 15/29 (51.7%) | 13/15 (86.7%) 13/29 (44.8%) |
6/7 (85.7%) | 5/6 (83.3%) 5/7 (71.5%) |
20/50 (40%) |
| B. cucurbitae | 9/29 (31%) | 6/9 (66.7%) 6/29 (20.7%) |
2/2 (100%) | 2/2 (100%) 2/2 (100%) |
11/27 (40%) |
| B. dorsalis | 14/29 (48.3%) | 12/14 (85.7%) 12/29 (41.4%) |
4/4 (100%) | 4/4 (100%) 4/4 (100%) |
29/36 (80.5%) |
| B. tryoni | 19/29 (65.5%) | 17/19 (89.5%) 17/29 (58.6%) |
5/6 (83.3%) | 5/5 (100%) 5/6 (83.3%) |
32/53 (60%) |
|
Bactrocera genus |
57/116 (49.1%) |
48/57 (84.2%) 48/116 (41.4%) |
17/19 (89.5%) |
16/17 (94.1%) 16/19 (84.2%) |
92/166 (55.4%) |
| A. fraterculus | 5/29 (17.2%) | 2/5 (40%) 2/29 (6.9%) |
2/2 (100%) | 1/2 (50%) 1/2 (50%) |
8/9 (88.9%) |
| A. ludens | 7/29 (24.1%) | 5/7 (71.5%) 5/29 (17.2%) |
1/1 (100%) | 1/1 (100%) 1/1 (100%) |
11/9 (122%) |
| A. serpentina | 9/29 (31%) | 6/9 (66.7%) 6/29(20.7%) |
1/1 (100%) | 1/1 (100%) 1/1 (100%) |
8/9 (88.9%) |
| A. striata | 7/29 (24.1%) | 5/7 (71.5%) 5/29 (17.2%) |
1/1 (100%) | 1/1 (100%) 1/1 (100%) |
12/9 (133%) |
| Anastrepha genus | 28/116 (24.1%) |
18/28 (64.3%) 18/116 (15.5%) |
5/5 (100%) |
4/5 (80%) 4/5 (80%) |
39/36 (108.3%) |
| C. capitata | 8/29 (27.6%) | 7/8 (87.5%) 7/29 (24.1%) |
1/2 (50%) | 1/1 (100%) 1/2 (50%) |
2/3 (66.7%) |
| C. fasciventris | 12/29 (41.4%) | 9/12 (75%) 9/29 (31%) |
2/2 (100%) | 2/2 (100%) 2/2 (100%) |
12/24 (50%) |
| Ceratitis genus | 20/58 (34.5%) |
16/20 (80%) 16/58 (27.6%) |
3/4 (75%) |
3/3 (100%) 3/4 (75%) |
15/27 (55.5%) |
| R. cerasi | 8/29 (27.6%) |
4/8 (50%) 13.8% |
1/3 (33.3%) |
1/1 (100%) 1/3 (33.3%) |
2/3 (66.7%) |
Functional primer pairs: number of primer pairs that cross-amplified successfully; Expected size: number of primer pairs that cross-amplified and produced fragment of the expected size (calculated either in regard to total available primer pairs or in regard to primer pairs that successfully cross-amplified); Presence of SSR motif: number of cross-species amplification products that harboured a microsatellite motif; Presence of the expected motif: number of cross-species amplification products that harboured the expected microsatellite motif (calculated either in regard to total sequencing reactions or in regard to cross-species amplification products that harboured a microsatellite motif); Mean number of uninterrupted repeats: sum and comparison of the number of uninterrupted repeats for primer pairs that cross-amplified and harboured the expected motif. Dividend represents repeats in the new species, while divider stands for number of repeats in B. oleae