Figure 5.

Phenotype and differentiation capacity of mesenchymal stem cells derived from single cells. (A) Bone marrow cells triple-stained with CD56-FITC, MSCA-1-PE and CD271-APC were gated and sorted as described in Figure 2C. Single cells were sorted in 96-well plates and cultured for 12 days. The resulting mesenchymal stem cells colonies were transferred to T-25 flasks and cultured for 12 more days and stained with the indicated antibodies. Note the heterogeneous expression profiles. (B) Morphology of MSCA-1⁺CD56⁺ derived mesenchymal stem cells clones C1 and C2, and MSCA-1⁺CD56⁻ derived clones C12 and C13. (C) Osteogenic, adipogenic, and neuronal differentiation potential of mesenchymal stem cells derived from single MSCA-1⁺CD56⁻ and MSCA-1⁺CD56⁺ bone marrow cells. Note that the MSCA-1⁺CD56⁻ clone C13 gives rise to adipocytes but not osteoblasts.