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. 1996 Dec 24;93(26):15057–15062. doi: 10.1073/pnas.93.26.15057

Figure 1.

Figure 1

Binding of islet and RINm5F nuclear extracts to human insulin DNA sequences. EMSA was performed with nuclear extracts (NE), using as probes the wild-type human insulin sequence (Wt.Ins) and its mutated forms A (lanes 5 and 11) and B (lane 10). Competition with 50-fold excess unlabeled double-stranded oligonucleotides of wild-type (Wt; lanes 2 and 7), mutant A (MtA; lanes 3 and 8), and mutant B (MtB) sequences (lanes 4 and 9).