Figure 2.

Effect of glucose on luciferase activity in transiently transfected normal islets and βTC1 cells. Wt contains the wild-type human insulin promoter linked to the luciferase reporter gene. Mt is the promoter carrying the A → G mutation (see Fig. 1). Cells were cultured in medium supplemented with 2 mM (LG) or 20 mM (HG) glucose for 48 h, extracted, and assayed for luciferase activity. Results represent the mean of five (Wt) and four (Mt) independent experiments in cultured islets and three in βTC1 cells.