Figure 3.

Purification of the GSF. Proteins were extracted from RINm5F nuclei and chromatographed over heparin–agarose followed by DNA affinity columns. (A) Coomassie blue staining of the final purified fraction subjected to SDS gel electrophoresis and transferred onto a PVDF membrane. Two major bands, of 45 kDa and 26 kDa, were detected. (B) EMSA of protein fractions eluted from sliced PVFD membrane containing the purified material shown in A. The 45-kDa polypeptide corresponds to GSF. NE-Is, islet nuclear extracts.