Figure 5.

Tissue distribution of hGSF/IPF-1 mRNA. Total RNA from rat (R.Isl) and human islets (H.Isl), β-cell lines RIN, βTC1, and HIT, AR42J exocrine cells, glucagon-producing αTC1 cells, and Hela cells were reverse-transcribed. cDNAs were amplified by PCR using human primers complementary to the 5′ and 3′ ends of the hGSF/IPF-1 mRNA. A 0.88-kb fragment corresponding to the hGSF/IPF-1 coding region is shown (Upper). Primers complementary to 194 bp of the ribosomal L19 gene were used as control (Lower). Pl, hGSF/IPF-1 plasmid.