Figure 6.

Transactivation of the human insulin promoter. (A) Indicated amounts of CMV–hGSF were cotransfected with wild-type insulin–luciferase construct in rat islets and in βTC1 cells. Expression is presented as percentage of control. Each column gives mean ± SD of three to seven independent experiments. (B) Rat islets, αTC1, and βTC1 cells were cotransfected with 0.25 μg (islets) or 5 μg (cell lines) CMV–sGSF (sense orientation, hatched bars) or CMV-aGSF (antisense, open bars) and with the wild-type human insulin (Wt) or the mutated insulin (Mt) luciferase constructs. Forty-eight hours after transfection, cell extracts were tested for luciferase activity. Results represent mean of three independent experiments. Luciferase activity was normalized to internal control β-galactosidase value.