FIGURE 5 .

Regulation of Zip8 expression during terminal erythroid differentiation. (A) Relative Zip8 mRNA abundance in erythroid progenitor cells were measured after being cultured in the conditions described in Figure 3. Data are means ± SD, n = 4 (independent experiments). ***Different from EPO deprived (EPO–) cells, P < 0.001. (B) Western analysis for detection of the EPO-dependent Zip8 protein expression was conducted as in Figure 4B. Relative densitometric values of the 60-kDa and 120-kDa bands for Zip8 in the EPO-treated cells were 1.5-fold of their respective control levels after 9 h of incubation. (C) Specificity of the 60-kDa and 120-kDa bands was determined by preincubating the respective antibody with a Zip8 peptide solution.