TABLE 6.
Cytokine mRNA expression levels in splenocytes from guinea pigs fed (n-3) or (n-6) fatty acid diets for 3 wk1
| Stimulated with ConA
|
Stimulated with PPD
|
||||||
|---|---|---|---|---|---|---|---|
| (n-3) PUFA | (n-6) PUFA | (n-3) PUFA | (n-6) PUFA | ||||
| Fold induction2 | |||||||
| IFNγ | 165 ± 66.7 | 337.4 ± 150.3 | 272 ± 115.4 | 333 ± 150.1 | |||
| TNFα3 | 6.2 ± 2.4 | 3.6 ± 0.6 | 7.2 ± 1.5 | 5.9 ± 1 | |||
| IL-10 | 4.0 ± 1.3 | 10.0 ± 5.3 | 9.5 ± 3.2 | 23.5 ± 7.5 | |||
| TGFβ3 | 2.2 ± 0.5 | 3.9 ± 1.7 | 5.5 ± 2.8 | 2.5 ± 0.6 | |||
1
Values are means ± SEM, n = 8.
2
Threshold cycle values normalized to Hypoxanthine phophoribosyl transferase expression and to the mean of unstimulated cell cultures at time 0.
3
TNFα, tumor necrosis factor-α; TGFβ; transforming growth factor-β.